Comparison of three molecular techniques for typing Pseudomonas aeruginosa isolates in sputum samples from patients with cystic fibrosis.

COMPARISON OF THREE MOLECULAR TECHNIQUES FOR TYPING PSEUDOMONAS AERUGINOSA ISOLATES IN CYSTIC FIBROSIS SPUTUM Running title: Molecular typing of P. aeruginosa in CF

Investigating the Presence of Type IV Pilin Subgenus in Pseudomonas Aeruginosa Isolated from Clinical and Non-Clinical Samples

Introduction: The type IV Pilin is an important colonization factor for opportunistic pathogens of Pseudomonas aeruginosa, which plays a role in the formation of biofilms and binding to the host cells. Each type of Pilin is coded with a particular auxiliary gene. This specific relationship can be used as a therapeutic target for detecting Pseudomonas aeruginosa strains as well as its molecular ...

Isolation and Genetic Fingerprinting of Pseudomonas aeruginosa from Iranian Patients with Cystic Fibrosis Using RAPD-PCR

Sixty four Iranian patients with cystic fibrosis (CF) were studied for colonization with Pseudomonasaeruginosa. The patient’s age ranged between 2 months to 18 years old. Twenty one patients werecolonized, 15 with non-mucoid and 6 with mucoid strains of P. aeruginosa. The colonization rateincreased with age and the mucoid phenotype was only recovered from the older patients. A...

Molecular detection of an atypical, highly resistant, clonal Pseudomonas aeruginosa isolate in cystic fibrosis patients.

BACKGROUND The identification of Pseudomonas aeruginosa (P. aeruginosa) isolates in sputum from cystic fibrosis (CF) patients can be challenging due to the multitude of phenotypic changes isolates undergo during adaptation to the microenvironment of the CF lung. METHODS We report the occurrence of shared P. aeruginosa isolates which failed identification by phenotypic methodologies and requir...

Detection of Ampc and Extended-Spectrum Beta-Lactamases in Clinical Isolates of Pseudomonas Aeruginosa from Patients with Cystic Fibrosis

ABSTRACT        Background and Objectives: Pseudomonas aeruginosa is the most frequent opportunistic pathogen isolated from the sputum of patients with cystic fibrosis (CF). Resistance to β -lactam antibiotics may arise from over expression of the naturally occurring AmpC cephalosporinases or acquired extended-spectrum β-lactamases (ESBL). The aim of...

Antibiotic Susceptibility of Pseudomonas Aeruginosa Isolated from Cystic Fibrosis Patients

Abstract Background and Objective: Cystic fibrosis (CF) is an autosomal recessive genetic disease and Pseudomonas aeruginosa is one of the most common bacteria colonized in CF patients. Growing resistance of this bacterium to antibiotics now a day is a challenge of controlling infection in CF patient. In this study colonization of CF patients with Pseudomonas aeruginosa and antibiotic suscep...